Title Epidemiologija Cryptosporidium spp. u školjkaša iz akvakulture Title (english) Epidemiology of Cryptosporidium spp. in shellfish from aquaculture Author Nikolina Penović Mentor Ivona Mladineo (mentor)Mentor Slaven Jozić (komentor)Committee member Maja Krželj (predsjednik povjerenstva)Committee member Ivona Mladineo (član povjerenstva)Committee member Slaven Jozić (član povjerenstva)Granter University of Split Defense date and country 2019-06-06, Croatia Scientific / art field, BIOTECHNICAL SCIENCES Abstract U ovom diplomskom radu predstavljeni su rezultati istraživanja prisutnosti protozoarnog nametnika Cryptosporidium spp. i enterokoknih bakterija u sredozemne dagnje (Mytilus galloprovincialis) iz akvakulture. Jedinke korištene u istraživanju prikupljene su na uzgajalištu Stipan Jaz u Marini pokraj Trogira, ostavljene su dva dana u moru izloženom mikrobiološkom onečišćenju (u blizini Kaštel Sućurca), a potom su postavljene u tri pokusna bazena za pročišćavanje sa slobodnim protokom morske vode,
... More na Institutu za oceanografiju i ribarstvo u Splitu. Analizirano je 240 uzoraka, od čega 120 uzoraka škrgi i 120 uzoraka plašta dagnji, prikupljenih tijekom četiri vremenske točke: nulto stanje prilikom postavljanja u bazene, te nakon 12, 24 i 48 sati pročišćavanja. Cryptosporidium spp. analiziran je iz uzoraka škrgi i plašta dagnji ugniježđenom lančanom reakcijom polimerazom, međutim, bez pozitivnog rezultata. Na to je mogla utjecati vremensko razdoblje u kojem su školjkaši bili pod antropogenim utjecajem, jako niska koncentracija oocista pristunih u moru u Kaštel Sućurcu, te određeni kemijski i fizički čimbenici (amonijak, pH, salinitet, temperatura, sunčevo zračenje, nedostatak oborina). Istraživanje koncentracije enterokoknih bakterija dalo je očekivane rezultate iz kojih je vidljiva visoka početna koncentracija enterokoknih bakterija koja nakon 48 sati pročišćavanja značajno opada. Može se zaključiti da Cryptosporidium spp. nema, ili ga ima u tako malim količinama koje nisu dovoljne da se akumulira i dokaže u dagnji, te samim time ne predstavlja opasnost za čovjeka. Ujedno, dagnje se kroz 48 sati pročišćavanjem oslobode enterokoknih bakterija do razine koncentracija koje su prihvatljive za konzumaciju. Less Abstract (english) The results of research on the presence of protozoan Cryptosporidium spp. and enterococci in the Mediterranean mussels (Mytilus galloprovincialis) from aquaculture are presented in this thesis. Specimens used in this research were collected from the farming site Stipan Jaz in Marina near Trogir, left for two days in the marine environment exposed to microbiological contamination (nearby Kaštel Sućurac) and subsequently placed in the three experimental pools for purification with flow-through
... More seawater system, at the Institute of oceanography and fisheries, Split. In total 240 samples were processed; 120 gill and mantle samples collected during four time points: 0, 12, 24 and 48 h after placement in the experimental pools. The presence of Cryptosporidium spp. was analysed from gills and mantle using nested PCR, however with no positive result. This may be due to the insufficient time that mussels were held under the microbiological contamination in the marine environment, very low concentration of oocyst in the sea column in the Kaštel Sućurac, and/ or certain chemical and physical factors (ammonia, pH, salinity, temperature, sun radiation, luck of rainfall). Analysis of the concentration of enterococci gave the expected results that show a high initial concentration of enterococci, which after 48 hours of purification, significantly decreases. It can be concluded that Cryptosporidum spp. is not present or is present in very low quantity, being not enough to accumulate and become detectable in mussels, therefore not posing a risk to humans. In addition, after 48 hours held in purification system, mussels decrease their initial concentration of enteroccoci to acceptable concentrations. Less Keywords
Cryptosporidium spp
crijevni enterokoki
Mytilus galloprovincialis
PCR
Sredozemno more
Keywords (english)
Cryptosporidium spp
intestinal enterococci
Mytilus galloprovincialis
PCR
Mediterranean
Language croatian URN:NBN urn:nbn:hr:226:380413 Study programme Title: Marine Fishery Type of resource Text File origin Born digital Access conditions Open access Terms of use Public note Parazitologija morskih organizama Created on 2020-01-02 13:08:52
