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undergraduate thesis
Zeleni fluorescentni protein
2018. urn:nbn:hr:226:577363
Kerum, Antonia
University of Split
University Department of Marine Studies

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Kerum, A. (2018). Zeleni fluorescentni protein (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:226:577363

Kerum, Antonia. "Zeleni fluorescentni protein." Undergraduate thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:577363

Kerum, Antonia. "Zeleni fluorescentni protein." Undergraduate thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:577363

Kerum, A. (2018). 'Zeleni fluorescentni protein', Undergraduate thesis, University of Split, accessed 14 November 2024, https://urn.nsk.hr/urn:nbn:hr:226:577363

Kerum A. Zeleni fluorescentni protein [Undergraduate thesis]. Split: University of Split; 2018 [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:226:577363

A. Kerum, "Zeleni fluorescentni protein", Undergraduate thesis, University of Split, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:226:577363

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Metadata
TitleZeleni fluorescentni protein
Title (english)Green fluorescent protein
AuthorAntonia Kerum
MentorŽeljka Trumbić (mentor)
Committee memberZvjezdana Popović Perković (predsjednik povjerenstva)
Committee memberŽeljka Trumbić (član povjerenstva)
Committee memberMirela Petrić (član povjerenstva)
GranterUniversity of Split
(University Department of Marine Studies)
Split
Defense date and country2018-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu su predstavljene karakteristike i primjene zelenog fluorescentnog proteina. Zeleni fluorescentni protein (GFP, engl. green fluorescent protein) izoliran je iz meduze Aequorea victoria koja je rasprostranjena duž zapadne obale Sjeverne Amerike. GFP ima oblik cilindra, tzv. formu β-limenke. Ciklizacijskim reakcijama unutarnjih lanaca β ploče i aminokiselina stvara se kromofor koji daje boju proteinu. Različitim mutacijama stvarane su različite varijante odnosno derivati GFP-a pa imamo plavi fluorescentni protein, cijan fluorescentni protein i žuti fluorescentni protein. Primjena GFP-a je raznolika, pa se isti koristi u dijagnostici tumora, kao reporter transkripcijske regulacije, kao biosenzor, pri vizualizaciji proteina u stanici te u istraživanju primarnih staničnih funkcija. Zbog svoje široke primjene nedvojbeno je da će se daljnjim istraživanjima poboljšati karakteristike GFP-a i njegovih derivata i tako proširiti područje primjene.
Abstract (english)
In this thesis, characteristics and applications of green fluorescent protein are presented. The green fluorescent protein (GFP) is isolated from the jellyfish Aequorea victoria that is widespread along the west coast of North America. GFP has cylinder shape also referred to as β-can structure. Cyclization reactions between inward-facing sidechains and amino acids creates chromophore that gives protein it's colour. Different mutations made different derivatives of GFP so we have blue fluorescent protein, cyan fluorescent protein and yellow fluorescent protein. Applications of GFP are diverse, in tumour diagnostics, as transcriptional regulation reporter, biosensor, when visualizing proteins in the cell, in research of primary cell functions, among others. It is undoubtable that further research will improve characteristics of GFP and its derivatives and thus extend the scope of their application.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:226:577363
Study programmeTitle: Marine Biology and Ecology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije i ekologije mora (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije i ekologije mora)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-04-21 13:53:55