The neon flying squids belong to a very rare cephalopods of the Adriatic Sea whose biology is almost unknown. 'Flying squids' got their name because of the ability to jump out of the water, as young juvenile individuals often jump above the sea surface when escaping predators. To date, there are only four reported records of this species in the Adriatic. In September 2020, a total of 23 juveniles of flying squid were collected in the central eastern Adriatic, in the area of the island of Palagruža (N = 14), the island of Jabuka (N = 1) and the Korčula Channel (N = 8), thus providing the opportunity to better study biology and genetic diversity of these rare cephalopods of the Adriatic. Based on a small sample size, this paper describes the preliminary morphometric characteristics of the squid body and beak, as well as the length-weight relationship that showed isometric growth. Dorsal mantle length ranged from 65 to 152 mm, while body weight ranged from 7.6 to 89.8 g. The age of the collected individuals was determined by the statolith reading method, and the estimated mean age was 49.3 days. Phylogenetic analysis using nucleotide sequence of the mitochondrial cytochrome c oxidase subunit I (COI) gene showed that collected samples belong to the species Ommastrephes caroli (Furtado, 1887) which was recently, along with the remaining three species, recognized within the genus Ommastrephes d'Orbigny that has been for many years considered a monotypic cosmopolitan genus with a discontinuous distribution.