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master's thesis
Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)
2018. urn:nbn:hr:226:643212
Šeparović, Ana
University of Split
University Department of Marine Studies

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Šeparović, A. (2018). Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758) (Master's thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:226:643212

Šeparović, Ana. "Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)." Master's thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:643212

Šeparović, Ana. "Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)." Master's thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:643212

Šeparović, A. (2018). 'Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)', Master's thesis, University of Split, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:226:643212

Šeparović A. Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758) [Master's thesis]. Split: University of Split; 2018 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:226:643212

A. Šeparović, "Utjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)", Master's thesis, University of Split, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:226:643212

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Metadata
TitleUtjecaj pH i temperature na sadržaj domoične kiseline u brbavicama Venus verrucosa (Linnaeus, 1758)
Title (english)pH and temperature influence on the content of domoic acid in warty venus Venus verrucosa (Linnaeus, 1758)
AuthorAna Šeparović
MentorIvana Ujević (mentor)
Committee memberJure Brčić (predsjednik povjerenstva)
Committee memberFrane Škeljo (član povjerenstva)
Committee memberIvana Ujević (član povjerenstva)
GranterUniversity of Split
(University Department of Marine Studies)
Split
Defense date and country2018-03-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Domoična kiselina (DK) je neurotoksin i uzročnik je ASP (Amnesic Shellfish Poisoning) otrovanja ljudi. Kvantitativno određivanje domoične kiseline u školjkašima je važno u prevenciji trovanja ASP-om koje bi moglo nastati nakon konzumacije toksičnih školjkaša. U ovom istraživanju ispitana je stabilnost domoične kiseline u ekstraktu brbavica (Venus verrucosa) pri vrijednostima pH od približno 1 i 3 te temperaturama od 37oC i 90oC. Domoična kiselina je određivana metodom tekućinske kromatografije i masene spektrometrije (LC-MS/MS) u metanolskom ekstraktu ukupnog jestivog mekog tkiva brbavica. Rezultati su ukazali da temperatura ekstrahiranja domoične kiseline ne utječe značajno na stabilnost domoične kiseline, dok vrijednost pH utječe. Naime, ustanovljen je utjecaj nižeg pH (1,09 – 1,50) pri kojem srednja vrijednost iskorištenja domoične kiseline iznosi 72,1 %, dok pri pH 3 (3,01 – 3,35) iznosi 99,6 % .
Abstract (english)
Domoic acid (DA) is a neurotoxin that causes ASP poisoning (Amnesic Shellfish Poisoning). Quantitative determination of domoic acid in shellfish is important in the prevention of ASP poisoning that could occur after the consumption of toxic bivalve molluscs. In this study, the stability of domoic acid in clams (Venus verrucosa) extract was tested at pH values of approximately 1 and 3 and at 37oC and 90oC. Domoic acid was determined by liquid chromatography and mass spectrometry (LC-MS/MS) in a methanol extract of total edible shellfish soft tissue. Domoic acid extraction temperature does not significantly affect the stability of the domoic acid while the pH is affected. The effect of lower pH (1.09 - 1.50) was established at which the mean value of domoic acid recovery was 72.1%.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:226:643212
Study programmeTitle: Marine Fishery
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka morskog ribarstva (magistar/ magistra inženjer/inženjerka morskog ribarstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public notePredmet: Toksičnost školjkaša
Created on2018-11-30 13:01:43